Student Research - Biology
Kylee Schuler
Investigation of the Role of Tylosin to Induce Resistance to Macrolides in Selected Bacterial Isolates
Abstract: Antibiotic usage, particularly macrolides, in animal husbandry is steadily increasing. This practice may contribute to increased resistance in these drugs that are commonly used to treat human infections. We investigated tylosin, commonly used to treat animals, to induce resistance and to examine the potential for cross resistance; that is resistance to other macrloides (erythromycin, azithromycin for example). After baseline Minimum Inhibitory Concentrations (MIC) were determined for Staphylococcus aureus 9144, Staphylococcus epidermidis, Bacillus subtiles, and E.coli HB101, each strain was induced with tylosin (sub-MIC levels) for two weeks. The data indicate repeated use of tylosin conferred resistance to selected macrolides: Rizithromycin, Erythromycin, Azithromycin, Roxithromycin, and Clarithromycin. Future studies will examine the effect of the concentration of tylosin to induce greater resistance to macrolides.
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